Principal investigator: MATHIAS CHAMAILLARD

Integrating the intra-tumoral microbiome to promote understanding on tumor plasticity

The partners of Oncobiomics proposed to study the tumoral microbiome have a source of cancer heterogeneity in colon cancer. Their aim is to integrate the intra-tumoral microbiome heterogeneity in order to promote understanding on tumor plasticity and offers new therapeutic options.

Works that led up to the project

Some past studies from the network led up to this project. First, they found a high load of bacteria in colon cancer tumors when compared to non tumoral mucosa and healthy patient mucosa. Moreover, this load is linked to tumor stage. With vivo mice xenograft models, they identified that pks + bacteria induce have a tumor-promoting activity, inducing bigger tumors than other bacterial stems.

Applying digital microbiology to tumor plasticity

First, the network performs a strong characterization of a cohort of 100 colorectal resections and their 100 no tumor adjacent tissue. Samples include tumors from Grade I to grade IV. Characterization is performed at different levels:

  • Bacterial level by sequencing of 16s and by topographic analysis of bacterial infiltrate
  • Genomical level by exome sequencing
  • Transcriptomical
  • Inflammation level by Immunoscore-like analysis

Mathematic modeling

With the data gathered, the network will mathematically model the functional dependencies of microbiome and tumoral cell in order to propose new drug development ideas. New therapeutics targets will be validated with in vivoin vitro and ex vivo models.

Oncobiomics highlights a new alternative to the usual thinking about cancer heterogeneity. Such characterization of colon cancer microbiome heterogeneity and its integration with clinical and genomic aspect of the tumor will promote a new understanding we tumor plasticity and new ways of therapeutic solutions.

This French program is organized by ITMO Cancer, in collaboration with ITMO BCDE (Cell Biology, Development and Evolution) and ITMO Technologies for Health of the National Alliance for Life Sciences and Health (AVIESAN) with the National Cancer Institute (INCA) and Inserm within the framework of the Cancer Plan. Operational management is entrusted to Inserm.
It was launch in order to develop a critical mass of resources and skills in order to conduct interdisciplinary research projects in the field of functional heterogeneity of cellular tumor relations in their ecosystem: the "HTE Program".

Initiation of HTE Program